A review on High Performance Liquid Chromatography

Review Article

Abstract

High-Performance Liquid Chromatography (HPLC) stands as a pivotal qualitative and quantitative analytical method extensively applied in the estimation of pharmaceutical and biological samples. Recognized as the most versatile and widely employed separation technique, this review article is dedicated to comprehensively surveying the instrumentation aspects of HPLC. Originating from the pioneering work of Csaba Horvath in 1964, the article explores the evolution, advancements, and key components of HPLC instrumentation, shedding light on its instrumental intricacies and technological innovations that have contributed to its enduring significance in analytical sciences.  This review aims to offer researchers, scientists, and practitioners a valuable resource for understanding the technological intricacies that underpin the reliability and efficacy of HPLC in analytical endeavors. Through a historical lens and a contemporary viewpoint, the article showcases the instrumental landscape of HPLC, emphasizing its enduring significance and the continuous innovations that shape its trajectory in analytical sciences