A Review of Principles, Applications, and Recent Developments in HPTLC and HPLC

Review Article

Abstract

High-Performance Thin Layer Chromatography (HPTLC) and High-Performance Liquid Chromatography (HPLC) represent fundamental analytical techniques in modern chemical analysis. These sophisticated separation methods have revolutionized the field of analytical chemistry through their precision, reliability, and versatility. HPTLC, an enhancement of traditional thin-layer chromatography, offers advantages such as minimal sample preparation, simultaneous analysis of multiple samples, and cost-effectiveness. It has found extensive applications in pharmaceutical analysis, natural product research, and food quality assessment. HPLC, characterized by its high resolution, sensitivity, and automation capabilities, has become an indispensable tool in various industries, including pharmaceutical, environmental, and biochemical analyses. The technique employs different modes such as reverse phase, normal phase, and ion exchange, enabling the separation of complex mixtures with remarkable accuracy. Recent technological advancements have led to the development of ultra-high-performance liquid chromatography (UHPLC), offering enhanced speed and resolution. Both HPTLC and HPLC complement each other in analytical laboratories, with HPTLC providing rapid screening and HPLC offering detailed quantitative analysis. The continuous evolution of these techniques through technological innovations ensures their pivotal role in modern analytical chemistry.